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To minimize unintended cold exposure during routine handling, culture media used for passaging were prewarmed to 37C prior to use

Br J Pharmacol 1999 Mar

Sulfasalazine, a potent suppressor of lymphoma growth by inhibition of the x(c)- cystine transporter: a new action for an old drug

Almost always, the problem traces back to a simple mistake in the reconstitution or handling process

After three washes with DPBS for 5 min, the cells were fixed in 10% neutral buffered formalin for 50 min at 24 C, followed by three washes with DPBS

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